-
-
pCMV-myc-FRNK
Plasmid#74481PurposeThis plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).DepositorInsertFRNK (PTK2 Chicken)
UseTagsc-mycExpressionMammalianMutationFRNK is the C-terminal, noncatalytic domain of th…PromoterCMVAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-SNAP-MD-OCT4
Plasmid#115691PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)DepositorInsertSNAP-MD-OCT4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAflaB
Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAftsI
Plasmid#149563PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPa-c6MYCn
Plasmid#69242PurposeTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-c6MYCn
Plasmid#69243PurposeTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 hMOG-beta2-EGFP
Plasmid#160979PurposeExpresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cellsDepositorInsertHomo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192239Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3eDepositorInserteIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSrTA1662-toolkit
Plasmid#154372PurposeExpresses SrTA1662 in plantsDepositorInsertSrTA1662
UseTagsExpressionBacterial and PlantMutationPromoterNative promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192241Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192236Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192237Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192238Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKPY737
Plasmid#72673PurposeEncodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)DepositorInsertThr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
UseGateway entry cloneTagsExpressionMutationThr412Gly in CePheRS Alpha SubunitPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2m
Plasmid#208686PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only
