We narrowed to 5,817 results for: pcas

1. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Expression

   Mammalian

   Promoter

   CMV/Chick β-actin (CAG)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS 3xFLAG Mbnl2

   Plasmid

   #217032

   Purpose

   Overexpression of Mbnl2 in mammalian cells

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Mbnl2 (Mbnl2 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-MBP-ATG9

   Plasmid

   #188076

   Purpose

   Recombinant overexpression of human ATG9 in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-ChrimsonR-mRuby2-ST

   Plasmid

   #105447

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Insert

   ChrimsonR-mRuby2-ST
   

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mammalian

   Mutation

   K176R in Chrimson

   Promoter

   pCAGGS

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG_Xph15-mNeonGreen-CCR5TC

   Plasmid

   #135533

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG2-EGFP-C-IFT121

   Plasmid

   #218736

   Purpose

   Expresses C-terminally EGFP-tagged IFT121 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT121 (WDR35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-Izumo1-1D4

   Plasmid

   #183544

   Purpose

   Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

   Insert

   izumo sperm-egg fusion 1 (Izumo1 Mouse)
   

   Tags

   1D4

   Expression

   Mammalian

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-ChroME-mRuby2-ST

   Plasmid

   #108902

   Purpose

   Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG/hArf4(WT)-mCherry

   Plasmid

   #79406

   Purpose

   Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pCAGGS-FLAG-CaMKIIB

    Plasmid

    #186826

    Purpose

    Expresses FLAG-CaMKIIB in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2b (Camk2b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-Scarlet-Kif5a

    Plasmid

    #118745

    Purpose

    Expresses scarlet tagged Kif5a

    Depositor

    Beverly Koller

    Insert

    Kif5a cDNA (Kif5a Mouse)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG mEmerald-LAMP1

    Plasmid

    #168513

    Purpose

    Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG2-EGFP-C-IFT139

    Plasmid

    #218738

    Purpose

    Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT139 (TTC21B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAP03-sp44/p21

    Plasmid

    #120232

    Purpose

    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-I-SceI-Trex2

    Plasmid

    #44024

    Depositor

    Jeremy Stark

    Article

    Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

    Insert

    I-SceI fused to mTREX2
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCARGO-RMCE-Xist

    Plasmid

    #233268

    Purpose

    CARGO plasmid containing ~22kb dox-inducible Xist transgene

    Depositor

    Mauro Calabrese

    Article

    Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

    Insert

    22kb Xist gene, including introns
    

    Expression

    Mammalian

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG2-EGFP-C-IFT122

    Plasmid

    #218737

    Purpose

    Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT122 (IFT122 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG_Xph15-SNAPf-CCR5TC

    Plasmid

    #135536

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Tags

    SNAPf

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge