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We narrowed to 5,878 results for: pcas

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Showing: 1101 - 1120 of 5878 results

  3. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  6. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172837-plasmid-map-sequence-id-340906

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  12. pCAG2-EGFP-C-IFT140

    Plasmid
    #218739
    Purpose
    Expresses C-terminally EGFP-tagged IFT140 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT140 (IFT140 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218739-plasmid-map-sequence-id-434233

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  14. pCAG-EGFP-C-IFT172

    Plasmid
    #218734
    Purpose
    Expresses N-terminally EGFP-tagged IFT172 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT172 (IFT172 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218734-plasmid-map-sequence-id-434222

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  19. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172836-plasmid-map-sequence-id-340903

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Showing: 1101 - 1120 of 5878 results