We narrowed to 1,209 results for: proc.2

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WNV NS2B-NS3 protease (catalytically active, self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204795

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204798

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBI-CMV1-mir144~451-CMV2-mir1

Plasmid

#164664

Purpose

Express miR-144~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204794

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Publication

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E2

Plasmid

#203831

Purpose

Luciferase reporter plasmid for Igf1 enhancer 2

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E2

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g2

Plasmid

#120210

Purpose

CENPB CRISPRi guide RNA 2

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-fixed-length-clone-2

Plasmid

#120180

Purpose

Randomly picked clone 2 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2-deltaTAR-luciferase

Plasmid

#215896

Purpose

Expression of luciferase

Depositor

Kumiko Ui-Tei

Publication

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

luciferase

Use

Luciferase

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Enterovirus A71 2A protease

Plasmid

#204812

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of Dengue2 NS2B-NS3 proteins

Plasmid

#204793

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET41-FeoB-OL2del

Plasmid

#216742

Purpose

Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.

Depositor

Gordon Laurie

Publication

Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

Insert

feoB (PA4358 P. aeruginosa (Bacteria))

Tags

8x His

Expression

Bacterial

Mutation

Outer loop 2 (OL2) with the sequence of [LEDSGYMA…

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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EV-A71 2A protease; 6xHis-tag, self cleaving

Plasmid

#204809

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; no tag, low expression levels

Plasmid

#204805

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pKMM019

Plasmid

#242507

Purpose

Derivative of pALC929 with a mutation (#2) in the RBS of OphK.

Depositor

Gregg Beckham

Publication

Mains et al Nature. 2026 Jun;654(8119):668-675. doi:

Inserts

Upstream homology arm
OphC_AG8816
OphC2_AG8816
OphK_AG8816
Downstream homology arm

Promoter

Ptac

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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pKMM020

Plasmid

#242508

Purpose

Derivative of pALC929 with a mutation (#3) in the RBS of OphK.

Depositor

Gregg Beckham

Publication

Mains et al Nature. 2026 Jun;654(8119):668-675. doi:

Inserts

Upstream homology arm
OphC_AG8816
OphC2_AG8816
OphK_AG8816
Downstream homology arm

Promoter

Ptac and tac

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; 6xHis-SUMO, low expression levels

Plasmid

#204802

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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