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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Publication

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Promoter

1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Publication

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Publication

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Gateway Cloning

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMS23: pHelper(PmcCAST)_entry_ΔTnsD

Plasmid

#168156

Purpose

Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_TMv2

Plasmid

#131761

Purpose

frameshift reporter for barcode retrieval

Depositor

Paul Blainey

Publication

Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

Plasmid

#140201

Purpose

Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

Depositor

Yit Heng Chooi

Publication

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Cloning of crrna an…

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-SCP1-ccdB

Plasmid

#131628

Purpose

Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

Depositor

Kevin White

Publication

Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

Type

Empty backbone

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SCP1

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_106

Plasmid

#130547

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-67Sma

Plasmid

#49342

Purpose

Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region

Depositor

Anastasios Melis

Publication

Chen et al Plant Biotechnol J. 2013 Sep;11(7):818-2

Insert

EcoRI 5.8 kB

Use

Synthetic Biology

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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Hs.HRAS G12V

Plasmid

#83184

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12V mutation

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

G12V

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DVK_pBR322_AE

Plasmid

#217592

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_AE

Plasmid

#217587

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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DVK_p15A_AF

Plasmid

#217583

Purpose

Destination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EC67904

Plasmid

#209470

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

HvU3

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70208

Plasmid

#209469

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC67895

Plasmid

#209506

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70200

Plasmid

#209461

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70189

Plasmid

#209450

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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