We narrowed to 17,592 results for: cript

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pGL3-Myc-rDMRdeltaTEAD

Plasmid

#235695

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Cyr61-rDMR

Plasmid

#235692

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Cyr61-rDMRdeltaTEAD

Plasmid

#235693

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Myc-rDMR

Plasmid

#235694

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_BbsI(GGA)

Plasmid

#231362

Purpose

minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED solvvol

Plasmid

#232958

Purpose

Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A

Plasmid

#231520

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutation at position 61 Q to A of the HMG d…

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A/E64M

Plasmid

#231521

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutations at position 61 Q to A and 64 E to…

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(GV12)

Plasmid

#233181

Purpose

Retroviral expression of HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

HRAS(G12V) (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1-P2A-mRuby2

Plasmid

#233169

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mLhx3-P2A-mRuby2

Plasmid

#233175

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-mNgn2x3HA-P2A-mRuby2

Plasmid

#233157

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Mouse, Synthetic)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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