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pHK302.5

Plasmid

#235470

Purpose

Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA5

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.1

Plasmid

#235460

Purpose

Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.3

Plasmid

#235462

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.1

Plasmid

#235449

Purpose

NOT gate receiver with binding site (bs) for sgRNA1

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.2

Plasmid

#235450

Purpose

NOT gate receiver with bs for sgRNA2

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.3

Plasmid

#235451

Purpose

NOT gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.4

Plasmid

#235452

Purpose

NOT gate receiver with bs for sgRNA4

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.5

Plasmid

#235453

Purpose

NOT gate receiver with bs for sgRNA5

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.6

Plasmid

#235454

Purpose

NOT gate receiver with bs for sgRNA6

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mStayGold-Hyg

Plasmid

#236486

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mStayGold-Nat

Plasmid

#236485

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP-U2-1

Plasmid

#236472

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

spH2Ap-3xmCherry spH2Bp-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mStayGold-G418

Plasmid

#236487

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mCherry-Nat

Plasmid

#236488

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-sfGFP-Hyg

Plasmid

#236480

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mCherry-G418

Plasmid

#236490

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mScarlet-Nat

Plasmid

#236491

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mCherry-Hyg

Plasmid

#236489

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-Dendra2-G418

Plasmid

#236496

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge