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pSKAP_AR

Plasmid

#122720

Purpose

pSKAP plasmid containing the unique DNA barcode "AR" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AR

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AU

Plasmid

#122723

Purpose

pSKAP plasmid containing the unique DNA barcode "AU" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AU

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AD

Plasmid

#122706

Purpose

pSKAP plasmid containing the unique DNA barcode "AD" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AD

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AC

Plasmid

#122705

Purpose

pSKAP plasmid containing the unique DNA barcode "AC" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AC

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AB

Plasmid

#122704

Purpose

pSKAP plasmid containing the unique DNA barcode "AB" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AB

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AA

Plasmid

#122703

Purpose

pSKAP plasmid containing the unique DNA barcode "AA" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AA

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AO

Plasmid

#122717

Purpose

pSKAP plasmid containing the unique DNA barcode "AO" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AO

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AI

Plasmid

#122711

Purpose

pSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AI

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AH

Plasmid

#122710

Purpose

pSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AH

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AG

Plasmid

#122709

Purpose

pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AG

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MetN

Plasmid

#114458

Purpose

Expression of hexahistidine-tagged AceS methylase in E. coli

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

aceS

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pORFE1001

Plasmid

#112079

Purpose

Overexpression of the AtCAS9 in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

2x35S

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Btk)KD(Akt1)

Plasmid

#86636

Purpose

Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)KD(Akt1)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTW087

Plasmid

#64756

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pUC120

Plasmid

#50350

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Type

Empty backbone

Use

Cloning vector

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pMOTag4HM-crelox-HYG

Plasmid

#24029

Depositor

George Cross

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA and 3XMyc

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO Cre v4 trcr filler

Plasmid

#256774

Purpose

lentiviral construct with Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.

Depositor

Daniel Schramek

Article

Al-Zahrani et al Nature. 2026 Jul 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

Lentiviral

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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