We narrowed to 14,458 results for: Cas9

Showing: 11261 - 11280 of 14458 results

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mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-SPI1

Plasmid

#162824

Purpose

Doxycycline-inducible overexpression of human SPI1

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC EJ7-GFP

Plasmid

#113617

Purpose

Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

EJ7-GFP variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUFM1

Plasmid

#86133

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFM1 (UFM1 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMK289 (mAID-mClover-NeoR)

Plasmid

#72827

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Neo

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBT44c

Plasmid

#122600

Purpose

BE PACE selection, express C-intein-Cas9-ugi fusion

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

intein-dCas9-ugi fusion

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-scramble

Plasmid

#206924

Purpose

Plasmid expressing Cas9, GFP and non-targeting guides for using as a control in CRISPR experiments

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

non-targeting human sgRNA guides

Use

CRISPR

Promoter

U6

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon5

Plasmid

#217456

Purpose

For TP53 knockout targeting exon 5 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon4

Plasmid

#217455

Purpose

For TP53 knockout targeting exon 4 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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