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pBABE-puro-mTwist

Plasmid

#1783

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

Twist (Twist1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

Plasmid

#202025

Purpose

Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)

Expression

Bacterial

Mutation

GvpB S9G-R31L-R85L

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Expression

Mammalian

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ENO1-Halo

Plasmid

#175330

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ENO1 (ENO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)MBII85

Plasmid

#67643

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYU-2-Env

Plasmid

#133997

Purpose

Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1786

Plasmid

#240489

Purpose

CRISPRi-mediated Knockdown of RPA1786

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pHA2

Plasmid

#85710

Purpose

This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDY1461_SERPINA1_CCA32_5avidity Cre

Plasmid

#193193

Purpose

Express Cre conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

Cre

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaPB

Plasmid

#124959

Purpose

Mammalian expression of Human TRIM28 with deleted PHD and BROMO domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of PHD and Bromo Domain

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP

Plasmid

#27704

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2

Plasmid

#86593

Purpose

Expresses eGFP-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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