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Showing: 11281 - 11300 of 19455 results

  1. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid
    #223167
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Publication
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223167-plasmid-map-sequence-id-442870

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  4. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid
    #193719
    Purpose
    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
    Tags
    2xHA-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193719-plasmid-map-sequence-id-383790

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  5. pSCM167.2-Venus-VF-hADAM10(aa455-550)

    Plasmid
    #189556
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 455-550
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189556-plasmid-map-sequence-id-384141

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  6. pSCM167-Venus-VF-hADAM10(aa220-459)

    Plasmid
    #189555
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 220-459
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189555-plasmid-map-sequence-id-374397

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  7. pSCM167.2-Venus-VF-hADAM10(aa551-654)

    Plasmid
    #189557
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa551-654
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189557-plasmid-map-sequence-id-374394

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  8. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

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  9. Venus-7aa-ActA-pEGFP-C1

    Plasmid
    #177405
    Purpose
    To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.
    Depositor
    David Andrews
    Publication
    Osterlund et al Protocol Exchange (2022)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    177405-plasmid-map-sequence-id-347977

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  10. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid
    #189561
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 455-654
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    189561-plasmid-map-sequence-id-374541

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  12. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Publication
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

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  13. pEGFP-N1-HSD17B11-S172L-FLAGC

    Plasmid
    #161904
    Purpose
    Expresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.
    Depositor
    Sébastien Britton
    Publication
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    Tags
    FLAG-GFP
    Expression
    Mammalian
    Mutation
    S172L; catalytically dead mutant
    Promoter
    CMV
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161904-plasmid-map-sequence-id-320523

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  14. p1.2-Zeo-eGFP

    Plasmid
    #162743
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Ivan Vorobiev
    Publication
    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162743-plasmid-map-sequence-id-339096

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  16. pGBW-m4136453

    Plasmid
    #149535
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149535-plasmid-map-sequence-id-313669

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  17. pGBW-m4095419

    Plasmid
    #149526
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    d1-1088 d1203-1945
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149526-plasmid-map-sequence-id-314689

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  18. pGBW-m4046950

    Plasmid
    #149258
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    149258-plasmid-map-sequence-id-358324

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  19. pGBW-m4046834

    Plasmid
    #149247
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149247-plasmid-map-sequence-id-358320

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  20. pGBW-m4046905

    Plasmid
    #145762
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145762-plasmid-map-sequence-id-307565

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Showing: 11281 - 11300 of 19455 results