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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHK002.4

Plasmid

#235463

Purpose

Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.4

Plasmid

#235469

Purpose

Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gHUS1_B

Plasmid

#211542

Purpose

sgRNA-B against HUS1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA HUS1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSC29

Plasmid

#104803

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Medtr4g094545

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-ATF7IPi1

Plasmid

#59614

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ATF7IPi2

Plasmid

#59615

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
OA-1050U (cinnabar)

Plasmid

#132423

Purpose

express arrays of gRNA targeting Cinnabar under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

cinnabar gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050V (wingless)

Plasmid

#132424

Purpose

express arrays of gRNA targeting Wingless under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

wingless gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo-MORC3_gRNA_3

Plasmid

#235530

Purpose

gRNA against human MORC3

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

MORC3 (MORC3 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNK928

Plasmid

#219736

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter del5_MtHP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of MtHP gene from Medicago truncatula, del5_MtHP

Use

Synthetic Biology

Expression

Plant

Promoter

del5_MtHP

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPGD guide 2

Plasmid

#193603

Purpose

PGD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPGD guide 2 (PGD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon3_1_As

Plasmid

#155057

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGCas9_scr

Plasmid

#207567

Purpose

GeoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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RcSxph E540A

Plasmid

#194019

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph E540D

Plasmid

#194020

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540D

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph E540Q

Plasmid

#194021

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540Q

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558A

Plasmid

#194022

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558F

Plasmid

#194023

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558F

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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