We narrowed to 32,780 results for: eng
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Plasmid#188309PurposeExpression of LuxE via a T7 promoter in TXTLDepositorInsertluxE
UseSynthetic BiologyExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsExpressionBacterialAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_E2At(2nd)
Plasmid#183085Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2At(2nd)
Plasmid#183086Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2At(2nd)
Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-2xL
Plasmid#186912PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)2-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-2xL
Plasmid#186908PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK035
Plasmid#180827PurposeT7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coliDepositorArticleInsertSwGdmB
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK036
Plasmid#180828PurposeT7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coliDepositorArticleInsertCnGdmA
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSB-FICAT_Payload-Esp3I
Plasmid#179380PurposeTransposon vector prepared for Esp3I golden gate cloning of TransgeneDepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only