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USP7 delta C-terminus

Plasmid

#234074

Purpose

Expresses USP7 mutant in E. coli

Depositor

Irina Bezsonova

Insert

USP7 1-1080 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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E547* FL USP7

Plasmid

#229001

Purpose

E547-mutant of human USP7

Depositor

Irina Bezsonova

Insert

USP7 1-546 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Usp5

Plasmid

#174873

Purpose

CRISPR vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Usp5 (Usp5 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.178

Plasmid

#184999

Purpose

Express -Eco1 FANCF editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

FANCF donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.176

Plasmid

#184997

Purpose

Express -Eco1 RNF2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

RNF2 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.179

Plasmid

#185000

Purpose

Express -Eco1 HEK4 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK4 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGK-Ldb3-mKate2

Plasmid

#128766

Purpose

Fluorescent mKate reporter for Ldb3

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ldb3 promoter (Ldb3 Mouse)

Expression

Mammalian

Promoter

Ldb3

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJB035

Plasmid

#22622

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB038

Plasmid

#22625

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB041

Plasmid

#22626

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Mutation

Site 3 mutation -117 to -116bp GG to cc

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB048

Plasmid

#22641

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pJB049

Plasmid

#22643

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Mutation

Site 5: CCACC to gagCg (-238 to -234bp)

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1328

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-hsp82 ts mutant (G170D) (HSP82 Budding Yeast)

Expression

Yeast

Mutation

G170D

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_EEF1A2_GTP-EFTU

Plasmid

#109862

Purpose

Protein expression and purification of EEF1A2_GTP-EFTU

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A2_GTP-EFTU (EEF1A2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSA109

Plasmid

#59783

Purpose

drives ZIF-1 protein in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
zif-1 coding sequence (zif-1 Nematode)
unc-54 3' UTR

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAX

Plasmid

#53274

Purpose

Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

Insert

crtE, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Mutation

Deleted idi (ORF6) and crtX genes

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DED/AAA USP7

Plasmid

#131253

Purpose

Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Contains point mutations that convert USP7 aspart…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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