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GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Expression

Mammalian

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-OptoSTIM1(CRY2clust)

Plasmid

#184715

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Publication

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0011

Plasmid

#205580

Purpose

NpuDnaE(C)_KAN(193-265)_RUBY

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0010

Plasmid

#205579

Purpose

KAN(1-192)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0009

Plasmid

#205578

Purpose

NpuDnaE(C)_KAN(132-265)_RUBY

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0008

Plasmid

#205577

Purpose

KAN(1-131)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Publication

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Expression

Bacterial

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_TatABmCherryC

Plasmid

#178545

Purpose

pTatABC with an mCherry fluorescent protein tag at the end of TatB.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmCherry, TatC

Tags

mCherry fused to TatB

Expression

Bacterial

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-Linker

Plasmid

#192305

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway Cloning

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+W275L

Plasmid

#196021

Purpose

Expresses CRKL W160L + W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Publication

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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