We narrowed to 7,572 results for: Lif;

1. pLV x EF1a_EKAREN4(TA)-ires-blast

   Plasmid

   #167831

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Desmoplakin II donor only control (FL-based no force control)

   Plasmid

   #118722

   Purpose

   The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   truncation after aa1353; Y72L mutation in mCherry…

   Promoter

   CMV

   Available since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

   Plasmid

   #194017

   Purpose

   Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(CTRL)
   eGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

   Plasmid

   #194015

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.1)
   eGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Desmoplakin II donor only control (F40-based tension sensor)

   Plasmid

   #118717

   Purpose

   The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1 GST-hUSP27X K181E

   Plasmid

   #225714

   Purpose

   Bacterial expression of USP27X (K181E) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X K181E (USP27X Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K181E

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS puro 3XFLAG-hATXN7L3

   Plasmid

   #225725

   Purpose

   Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   ATXN7L3 (ATXN7L3 Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS puro HA-hUSP27X S404N

   Plasmid

   #225724

   Purpose

   Transfection of USP27X (S404N) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X S404N (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S404N

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS puro HA-hUSP27X Y381H

   Plasmid

   #225723

   Purpose

   Transfection of USP27X (Y381H) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y381H

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS puro HA-hUSP27X F313V

    Plasmid

    #225722

    Purpose

    Transfection of USP27X (F313V) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X F313V (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F313V

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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