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CDK11B gRNA (BRDN0001146017)

Plasmid

#77066

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Mustard Weed, Human)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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AG0058 LGA (DIO-mGlp1r-S33W)

Plasmid

#249036

Purpose

Expression of Mutant Mouse Glp1r-S33W, Cre dependent under hSyn promoter

Depositor

Ali Guler

Article

Godschall et al bioRxiv [Preprint]. 2025 Jun 14:2024.12.

Insert

mGlp1r (S33W)

Use

AAV

Tags

FLAG and HA

Mutation

33S to 33W

Promoter

hSyn

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

Plasmid

#245377

Purpose

Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

Depositor

D. James Surmeier

Article

Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

3xFLAG, T2A-GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1 T62 H67 M68

Plasmid

#246506

Purpose

Recombinant protein expression of PCMTD1 T62 H67 M68

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

Plasmid

#240228

Purpose

Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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synapsin-hIRβ-mCherry

Plasmid

#228449

Purpose

Expressing a truncated, constitutively active human insulin receptor (IRβ) with mCherry in rat primary hippocampal neurons

Depositor

Olivier Thibault

Article

Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

Insert

human Insulin Receptor beta subunit (INSR Human)

Use

AAV

Promoter

neuron-specific synapsin promote

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-TAZ-CAMTA1

Plasmid

#235681

Purpose

Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

Depositor

Duojia Pan

Article

Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

Insert

TAZ-CAMTA1 (WWTR1, CAMTA1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW D262V

Plasmid

#234616

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW D262N

Plasmid

#234615

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

Plasmid

#212402

Purpose

Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F,D614G,Q677H

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-6 [N270/28R]

Plasmid

#225385

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse) IgG2a R-mAb. Derived from hybridoma N270/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-6 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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