We narrowed to 60,545 results for: tra
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Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab2WT
Plasmid#46779Purposeexpression of WT Rab2a in mammalian cellsDepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
hMTP
Plasmid#124281PurposeExpresses human MTP in mammalian cells.DepositorAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
Tags6XHIS tagExpressionBacterialPromoterT7Available SinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
ExpressionWormPromoterC. elegans rab-3 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)PromotertetA promoter of plasmid pACYC184 originAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eNOS-GFP
Plasmid#22444DepositorInsertWT eNOS-GFP
TagsGFPExpressionMammalianAvailable SinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only