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TRBV15

Plasmid

#237940

Purpose

Encodes TRBV15 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV15-TRAC (TRBV15 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV10-3

Plasmid

#237931

Purpose

Encodes TRBV10-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV10-3-TRAC (TRBV10-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV11-1

Plasmid

#237932

Purpose

Encodes TRBV11-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV11-1-TRAC (TRBV11-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-9

Plasmid

#237927

Purpose

Encodes TRBV7-9 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV7-9-TRAC (TRBV7-9 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV9

Plasmid

#237928

Purpose

Encodes TRBV9 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV9-TRAC (TRBV9 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV10-1

Plasmid

#237929

Purpose

Encodes TRBV10-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV10-1-TRAC (TRBV10-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-8

Plasmid

#237919

Purpose

Encodes TRBV6-8 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV6-8-TRAC (TRBV6-8 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV2

Plasmid

#237903

Purpose

Encodes TRBV2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV2-TRAC (TRBV2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV4-2

Plasmid

#237906

Purpose

Encodes TRBV4-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV4-2-TRAC (TRBV4-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

Plasmid

#229831

Purpose

A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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macroH2A1.1-F192V-GFP (pc5114)

Plasmid

#223437

Purpose

Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-F192V (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

Plasmid

#217685

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

CELSR2 (CELSR2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2356

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

Plasmid

#217710

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRV1 (ADGRV1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5890

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTF-FLAG

Plasmid

#217700

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-606

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-F2353D_W

Plasmid

#147883

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-F2353D

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1833-2361-F2353D (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-I2330E_W

Plasmid

#147884

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-I2330E

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1833-2361-I2330E (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT-RP2.3

Plasmid

#189843

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

GFP GM2 variant and mRFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT-RP8.3

Plasmid

#189848

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

mRFP and tagBFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_EGFP-P2A_truncHaus6_IRES_Blast

Plasmid

#182885

Purpose

Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

Depositor

Daniel Gerlich

Article

David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

Insert

HAUS 6 (HAUS6 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

stop codon introduced 2 amino acids downstream of…

Promoter

EF1alpha core

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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