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Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
UseTagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
enz.083-NMT1
Plasmid#154047PurposeExpresses NMT1 in E. coliDepositorInsertNMT1 (N-myristoyltransferase 1) (NMT1 Human)
UseTagsHis, MBPExpressionBacterialMutationPromoterT7Available sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSHS293
Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTMS1
Plasmid#87799PurposeFor constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTMS2
Plasmid#87800PurposeFor constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-AvrPWT4
Plasmid#237522PurposeRecombinant protein expressionDepositorInsertAvrPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-RWT4Ca
Plasmid#237519PurposeRecombinant protein expressionDepositorInsertRwt4Ca
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-VirPWT4
Plasmid#237523PurposeRecombinant protein expressionDepositorInsertVirPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySIM
Plasmid#221068PurposeExpression of polySIM protein under T7 promoterDepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
UseTagsHis and MBPExpressionBacterialMutationPromoterunknownAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_K262A
Plasmid#225728PurposeTo express Mce3R mutant K262A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationK262APromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T_Mce3R
Plasmid#225726PurposeTo express Mce3R in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only