We narrowed to 2,695 results for: GUS

1. pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

   Plasmid

   #187331

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

   Plasmid

   #195537

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

   Plasmid

   #202058

   Purpose

   Inducible AAV with reporter gene and cloning site to add activator domains

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-noActivator Domain -Citrine
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITRs intact

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

   Plasmid

   #202061

   Purpose

   Inducible AAV activator

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITRs intact

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

   Plasmid

   #159290

   Purpose

   Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT6A (ID. A8) (KAT6A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_EEF1A2_GTP-EFTU-D2

   Plasmid

   #109919

   Purpose

   Protein expression and purification of EEF1A2_GTP-EFTU-D2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EEF1A2_GTP-EFTU-D2 (EEF1A2, EEF1A1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W

   Plasmid

   #159289

   Purpose

   Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT6A (ID. A7) (KAT6A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65429

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

   Plasmid

   #187334

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMV4 lenti pEF-rTetR(SE-G72P)-3XFLAG-ANM2(SH3_1)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188750

    Purpose

    Lentiviral backbone containing ANM2 SH3_1 domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ANM2 SH3_1 (PRMT2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CS2 Flag-Smad2 (3A)

    Plasmid

    #101763

    Purpose

    Expresses SMAD2 (3A) in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SMAD2 (SMAD2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Three Ser to Ala mutations in the phosphorylation…

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65454

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187337

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #187324

    Purpose

    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188757

    Purpose

    Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF10 KRAB (ZNF10 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188755

    Purpose

    Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NOTC2 Notch (NOTCH2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Pvalb-2A-Dre Flp-in replacement vector

    Plasmid

    #61571

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188756

    Purpose

    Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    WWP2 WW (WWP2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188759

    Purpose

    Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF473 KRAB (ZNF473 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMV8 lenti pEF-rTetR(SE-G72P)-3XFLAG-PYGO1(PHD)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188754

    Purpose

    Lentiviral backbone containing PYGO1 PHD domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    PYGO1 PHD (PYGO1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge