We narrowed to 3,495 results for: Gla

1. pET21-pelB-LaM-6

   Plasmid

   #172771

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-6
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRESpuro2-UBXD1-3x HA

   Plasmid

   #31836

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   UBXD1 (UBXN6 Human)
   

   Use

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5FRT/TO-p97-EQ-mycStrep

   Plasmid

   #31838

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   E578Q

   Promoter

   CMV

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-rap1-K9R

   Plasmid

   #84527

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   none

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5FRT/TO-p97-AE-mycStrep

   Plasmid

   #31841

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   A232E

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaG-8

   Plasmid

   #172761

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmalc4x_I499F_TEV

    Plasmid

    #47703

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with I499F mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed I499F

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pXD71Cas10.1-Pct5.1-crRNA(dadR)

    Plasmid

    #191634

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/TO-p97-RH-mycStrep

    Plasmid

    #31839

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R155H

    Promoter

    CMV

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSW002-Pc-TorT(sp)

    Plasmid

    #111255

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT signal peptide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-p97-RG-mycStrep

    Plasmid

    #31840

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R95G

    Promoter

    CMV

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

18. (369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

    Plasmid

    #168190

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-RAP1

    Plasmid

    #84525

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    none

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

    Plasmid

    #110747

    Purpose

    Bacterial expression of human USP30 (construct 13i)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    C77A, F348D, M350S, I353E + insertion deletion: 6…

    Promoter

    T7

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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