We narrowed to 2,171 results for: Xpa

1. pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

   Plasmid

   #244838

   Purpose

   Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-Cas12a
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q

   Promoter

   T7

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHyg-AID*-GFP

   Plasmid

   #99521

   Purpose

   C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   GFP

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-G-GECO1.2

   Plasmid

   #32446

   Purpose

   Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-tetO-MCS

   Plasmid

   #84008

   Purpose

   doxycycline-inducible lentiviral vector

   Depositor

   Stefano Piccolo

   Article

   Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYDS2.0_AT118-L

   Plasmid

   #222847

   Purpose

   Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Expression

   Yeast

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32a-TRXtag

   Plasmid

   #11516

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   None - thioredoxin is vector encoded (trxA )
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   vector modified; removed N-terminal His tag by di…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

8. CMV-B-GECO1

   Plasmid

   #32448

   Purpose

   Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   B-GECO1.0
   

   Expression

   Mammalian

   Mutation

   GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

   Promoter

   CMV

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPLICS Po-Mt Short P2A

   Plasmid

   #164116

   Purpose

   Detect the short Peroxisomes-mitochondria contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Short P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGF-mSox2-ONL

    Plasmid

    #65719

    Purpose

    mSox2 reporter plasmid (expresses ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Sox2 promoter and ONL
    

    Use

    Lentiviral and Luciferase

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNat-AID*-9myc

    Plasmid

    #99523

    Purpose

    C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ND4.2-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #179683

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    E1347A

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-RdLight1

    Plasmid

    #125706

    Purpose

    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Insert

    RdLight1
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEC3006 (pSpy1C-PZn_ffluc)

    Plasmid

    #218515

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PZn (PczcD)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138129

    Purpose

    Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-rabbit-IgG(Fc)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHis-AID*-9myc

    Plasmid

    #99524

    Purpose

    C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMALB

    Plasmid

    #161786

    Purpose

    Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    Bidirectional minhCMV and SFFV

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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