We narrowed to 1,998 results for: dox

1. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-off TauRD (P301L/V337M)

   Plasmid

   #188572

   Purpose

   Dox-repressible expression of the Tau repeat domain

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   Tau repeat domain (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-MCU-Flag-TetOFF

   Plasmid

   #185657

   Purpose

   Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE3G-GSX2-IRES2-EBF1

   Plasmid

   #96966

   Purpose

   Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
   

   Use

   Tags

   FLAG (on EBF1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156430

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Cterm_Nterm_switched

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

   Plasmid

   #70076

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Silent mutations are introduced to escape from RN…

   Promoter

   Tet responsible promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156434

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   All zinc fingers mutated

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid

    #70075

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Y102A. Silent mutations are introduced to escape …

    Promoter

    Tet responsible promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSITEP-shBIRC6-2(3)

    Plasmid

    #202451

    Purpose

    DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSITEP-shBIRC6-2

    Plasmid

    #202449

    Purpose

    DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-HAND2

    Plasmid

    #170690

    Purpose

    doxycycline-inducible overexpression of HAND2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1-V16-Luc

    Plasmid

    #122034

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1 blast OFF 3xflag IREB2

    Plasmid

    #169889

    Purpose

    Express 3x flag IREB2, suppressible by doxycycline addition

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    IREB2 (IREB2 Human)
    

    Use

    Lentiviral; Doxycycline mediated suppression

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

    Promoter

    TRE

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICE-HA-Ku70-siR-Mut6E

    Plasmid

    #82330

    Purpose

    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mut6E corresponding to K282E K287E T300E K331E K3…

    Promoter

    CMV-tet

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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