We narrowed to 14,409 results for: eng
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Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorPublicationInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVEC1
Plasmid#241910PurposePrimary screening of PET hydrolases and enzyme condition screening. TphR-based terephthalate (TPA) biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttphR, tphK, sfGFP
UseSynthetic BiologyMutationnoneAvailable sinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB1K3 containing mRFP1
Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpromoter, RBS, mRFP1
UseEscherichia coliAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
ExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsExpressionBacterialPromoterPgyrAAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-eeBxb1
Plasmid#232748PurposeIVT template for making modRNA encoding eeBxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSMutationV74A, E229K, V375IAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pgRNA
Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7070
Plasmid#214008PurposePrpsM driven expression of ssrA-tagged mNeonGreen (ASV)DepositorInsertmNeonGreen-ASV
ExpressionBacterialPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM8731
Plasmid#213991PurposePrpsM driven expression of mNeonGreen - sequence codon-optimized for SalmonellaDepositorInsertmNeonGreen
ExpressionBacterialPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM10686
Plasmid#213995PurposePrpsM driven expression of mScarlet-IDepositorInsertmScarlet-I
ExpressionBacterialPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SE
Plasmid#52140PurposeEncodes module 1 with amino acids 12S-16E for recognition of nt 8GDepositorPublicationInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 16Q to 16E in Module 1Available sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SE
Plasmid#52144PurposeEncodes module 2 with amino acids 12S-16E for recognition of nt 7GDepositorPublicationInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 2Available sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SE
Plasmid#52148PurposeEncodes module 3 with amino acids 12S-16E for recognition of nt 6GDepositorPublicationInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 3Available sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYR
Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorPublicationInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…Available sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SR
Plasmid#52162PurposeEncodes module 5 with amino acids 12S-16R for recognition of nt 4CDepositorPublicationInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 5Available sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only