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pLenti-CMV-TMPRSS2-TwinStrep_Zeo

Plasmid

#192000

Purpose

Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

TMPRSS2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF804B

Plasmid

#88756

Purpose

Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF804B (ZNF804B Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXQ1

Plasmid

#101628

Purpose

Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXQ1 (FOXQ1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pINSPECT:IL2_SigP:NLuc

Plasmid

#197266

Purpose

Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Insert

Interleukin-2 homology arms

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pINSPECT:NEAT1-v2_SigP:NLuc

Plasmid

#197268

Purpose

Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Insert

NEAT1-v2 homology arms

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_FLuc_loxP-PuroR

Plasmid

#177866

Purpose

Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

FLuc-based EXSISERS

Use

CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-HES3

Plasmid

#101411

Purpose

Donor Vector containing HES3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HES3 (HES3 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-mEtfdh-AAA4

Plasmid

#232316

Purpose

Expression of mouse mutant Etfdh (AAA4)

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh (Etfdh Mouse)

Use

Lentiviral

Tags

Expression

Mutation

PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pINSPECT:NEAT1-total_SigP:NLuc

Plasmid

#197267

Purpose

Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Insert

NEAT1-total homology arms

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_NLuc_FRT-PuroR

Plasmid

#177865

Purpose

Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

NLuc-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_BSD_FRT-PuroR

Plasmid

#177867

Purpose

Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

BSD-based EXSISERS

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_SurfaceHaloTag_FRT-PuroR

Plasmid

#177869

Purpose

Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

Surface-HaloTag-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00001

Plasmid

#166718

Purpose

sgRNA against human BAP1

Depositor

Raj Chari

Article

Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

Insert

BAP1 (BAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00002

Plasmid

#166719

Purpose

sgRNA against human BAP1

Depositor

Raj Chari

Article

Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

Insert

BAP1 (BAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-FOXI3

Plasmid

#101518

Purpose

Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXI3 (FOXI3 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ASCL3

Plasmid

#101515

Purpose

Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ASCL3 (ASCL3 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HOXB3

Plasmid

#101478

Purpose

Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB3 (HOXB3 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-FOXE1

Plasmid

#101439

Purpose

Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE1 (FOXE1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge