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pMSCV Cdk2ap1ΔN (MT2B2)-HA

Plasmid

#178031

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI

Plasmid

#148949

Purpose

Mammalian Expression of HsGIGYF2_1-718-del280-310

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718-del280-310 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-6 [N270/47R]

Plasmid

#188216

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse). Derived from hybridoma N270/47

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-6 (Mus musculus) recombinant mouse monoclonal antibody (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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EIF2B3-S369fs

Plasmid

#172089

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1106_1113del

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange-imp α

Plasmid

#119720

Purpose

Expresses mOrange-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mOrange

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-A569T

Plasmid

#116575

Purpose

Lentiviral expression of PIK3CG A569T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

A569T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-D192G

Plasmid

#116576

Purpose

Lentiviral expression of PIK3CG D192G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

D192G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-G426E

Plasmid

#116579

Purpose

Lentiviral expression of PIK3CG G426E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

G426E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-R130Q

Plasmid

#116581

Purpose

Lentiviral expression of PIK3CG R130Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

R130Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-S806F

Plasmid

#116583

Purpose

Lentiviral expression of PIK3CG S806F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

S806F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R604Gfs*4

Plasmid

#116560

Purpose

Lentiviral expression of PIK3CB R604Gfs*4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

R604Gfs*4

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R604Pfs*29

Plasmid

#116561

Purpose

Lentiviral expression of PIK3CB R604Pfs*29

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

R604Pfs*29

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Expression

Plant

Promoter

RCH1

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_PIK3CB_p.E497D

Plasmid

#81599

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

E497D; Q759R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_p.A593V

Plasmid

#81602

Purpose

Gateway Donor vector containing PIK3CB ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

A593V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_p.A1048V

Plasmid

#81533

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

A1048V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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DGKZ gRNA (BRDN0001147837)

Plasmid

#77464

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKZ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKZ (DGKZ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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