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pSliceRep_A_miR-196a

Plasmid

#220466

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1198

Plasmid

#219740

Purpose

MoClo-compatible Level 0 - 3U + terminator vector carrying terminator HSP18.2

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

The heat shock protein 18.2 (HSP) terminator

Use

Synthetic Biology

Expression

Plant

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11-B5

Plasmid

#184279

Purpose

Expresses nanobody H11-B5 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-B5

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_11-H6

Plasmid

#184280

Purpose

Expresses nanobody H11-H6 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H6

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_8xTEAD_luc2

Plasmid

#173005

Purpose

firefly luciferase reporter vector with 8x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

8xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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RS02060_pMQ30_KO_Kan

Plasmid

#185391

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS02060 upstream flanking homology region
RR42_RS02060 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS28935_pMQ30_KO_Kan

Plasmid

#185392

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS28935 upstream flanking homology region
RR42_RS28935 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18965_pMQ30_KO_Kan

Plasmid

#185394

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18965 upstream flanking homology region
RR42_RS18965 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18970_pMQ30_KO_Kan

Plasmid

#185395

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18970 upstream flanking homology region
RR42_RS18970 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17060_pMQ30_KO_Kan

Plasmid

#185396

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17055_pMQ30_KO_Kan

Plasmid

#185398

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)

Plasmid

#203542

Purpose

Make FIP200 NTD:ATG13:ULK1 complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-mNeonC

Plasmid

#178461

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2572

Plasmid

#195683

Purpose

Expression/purification of 6xHis-TEV-MlaC(V171R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(V171R)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1223

Plasmid

#195688

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta173-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta173-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1909

Plasmid

#195690

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1910

Plasmid

#195691

Purpose

Expression/purification of 6xHis-TEV-MlaD(L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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