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pKanCMV-mRuby3-18aa-Tubulin

Plasmid

#74256

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAB088c

Plasmid

#79213

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pZY102

Plasmid

#73933

Purpose

This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Inserts

bar
GUS

Expression

Plant

Mutation

intron is inserted into the GUS coding region

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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GFP HDAC5 WT

Plasmid

#32211

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pKanCMV-mClover3-10aa-H2B

Plasmid

#74257

Purpose

Mammalian expression of N-terminal fusion of mClover3 to H2B

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Tags

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

actin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-Tubulin

Plasmid

#74253

Purpose

Mammalian expression of N-terminal fusion of mClover3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)

Plasmid

#199165

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-DHB-mTurq2

Plasmid

#212667

Purpose

Express tagged DHB

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

DHB (HELB Human)

Use

Lentiviral

Tags

mTurq2

Promoter

EF-1a

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX304-mNeonGreen

Plasmid

#162034

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Neon Green

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LZRS-hNEDD9_IresGFP

Plasmid

#21962

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

NEDD9 (NEDD9 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pLAMP1-emiRFP670

Plasmid

#136570

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LAMP1-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Article

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE347

Plasmid

#153228

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 MDC123

Plasmid

#59184

Purpose

2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

2X35S

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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