We narrowed to 20,611 results for: ATO

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pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

Plasmid

#191642

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Tags

3xFLAG

Expression

Bacterial

Mutation

ΔTM

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

Plasmid

#191643

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Tags

3xFLAG

Expression

Bacterial

Mutation

Linker region sequence shuffled

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadH)-Pdadh-DadhABC(A2)

Plasmid

#191644

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160a

Plasmid

#173756

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160a_sgRNA2/R160a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160b

Plasmid

#173757

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160b_sgRNA2/160b

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R390a

Plasmid

#173758

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/390a_sgRNA2/390a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N2

Plasmid

#174001

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O2

Plasmid

#174002

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-E (aa1-138)-EGFP

Plasmid

#187697

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa1-138

Promoter

CMV

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-G (aa1-372)-EGFP

Plasmid

#187699

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa1-372

Promoter

CMV

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-F (aa373-564)-EGFP

Plasmid

#187698

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa373-564

Promoter

CMV

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-C (aa139-371)-EGFP

Plasmid

#187695

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa139-371

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-A (aa139-564)-EGFP

Plasmid

#187693

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa139-564

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-B (aa309-564)-EGFP

Plasmid

#187694

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa309-564

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTE1069

Plasmid

#186629

Purpose

SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbR
scbAp promoter

Use

Synthetic Biology

Tags

6xArg

Expression

Bacterial

Promoter

BBa_J23100 and scbAp

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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