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pDL23_Cas9-His Δcys E945C

Plasmid

#206284

Purpose

Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

Depositor

Amit Choudhary

Publication

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E945C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL24_Cas9-His Δcys E1026C

Plasmid

#206285

Purpose

Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

Depositor

Amit Choudhary

Publication

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1026C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL22_Cas9-His Δcys E532C

Plasmid

#206283

Purpose

Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

Depositor

Amit Choudhary

Publication

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-T2A-EGFP

Plasmid

#188271

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX-T2A-EGFP

Plasmid

#188273

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-Cre-StLS1-9

Plasmid

#197722

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Cre recombinase with St-LS1 intron (maize optimized)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3-F672AY676AF727A_S

Plasmid

#147505

Purpose

Mammalian Expression of HsNot3-F672AY676AF727A

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-F672AY676AF727A (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3_1-241_K

Plasmid

#146795

Purpose

Mammalian Expression of HsNot3_1-241

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_1-241 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3_589-753_K

Plasmid

#146797

Purpose

Mammalian Expression of HsNot3_589-753

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_589-753 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Ccu-sgRNA-SV40-puro

Plasmid

#192130

Purpose

Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Publication

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Ccu-SgRNA scaffold

Expression

Mammalian

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Hsp1-sgRNA-SV40-puro

Plasmid

#192129

Purpose

Expresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Publication

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-SgRNA scaffold

Expression

Mammalian

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1163A

Plasmid

#194001

Purpose

pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

Depositor

Omar Akbari

Publication

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

Uniq-CasRx (Codon optimized)

Expression

Insect

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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H1.224.luc

Plasmid

#193704

Purpose

Expresses a truncated PolII H1 promoter (H1.244)

Depositor

Elena Herrera-Carrillo

Publication

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment

Use

Luciferase

Expression

Mammalian

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pIALD2E

Plasmid

#185866

Purpose

Knocking out ALD6

Depositor

Claudia Vickers

Publication

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD(-125, 40)- ALD6(1054, 1749)

Expression

Yeast

Mutation

None

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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sCatch-GFP-6xHIS

Plasmid

#186904

Purpose

Bacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tag

Depositor

Taekjip Ha, Allen Liu

Publication

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

sfGFP-SpyCatcher003-sfCherry1-10-6xHIS

Tags

6xHis and sfGFP

Expression

Bacterial

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-742pUC19-tNGFR-P2A-Caspase8

Plasmid

#186099

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CASPASE8

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNB203

Plasmid

#169114

Purpose

TESEC expression vector for mEGFP-Alr fusion construct

Depositor

Edwin Wintermute

Publication

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB205

Plasmid

#169116

Purpose

TESEC expression vector for Asd from M. tuberculosis

Depositor

Edwin Wintermute

Publication

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

asd (asd Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB206

Plasmid

#169117

Purpose

TESEC expression vector for CysH from M. tuberculosis

Depositor

Edwin Wintermute

Publication

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

cysH (cysH Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB207

Plasmid

#169118

Purpose

TESEC expression vector for DapB from M. tuberculosis

Depositor

Edwin Wintermute

Publication

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

dapB (dapB Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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