We narrowed to 59,231 results for: %s

1. pDECKO_mCherry_Rat_cebp-alfa

   Plasmid

   #140098

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDECKO_mCherry_SPI1

   Plasmid

   #140099

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmirGLO-Bmpr2-Δ17

   Plasmid

   #49380

   Purpose

   Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Bmpr2 (BMPR2 Human, Mouse)
   

   Use

   Luciferase

   Mutation

   changed nucleotides AC to TG at bp 7347-7348

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET-A2-77p-Venus

   Plasmid

   #64856

   Purpose

   Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

   Depositor

   Sriram Neelamegham

   Article

   Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

   Insert

   VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)
   

   Tags

   7X His and Venus

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRShygro shID3#1

   Plasmid

   #19164

   Depositor

   Joan Massague

   Article

   Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

   Insert

   small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLV V5-nurim

   Plasmid

   #175110

   Purpose

   Lentiviral expression of V5-tagged mouse Nrm

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Nrm (Nrm Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3/flag-pBirA(1-G78)-FRB

   Plasmid

   #154447

   Purpose

   expresses pBirA(1-G78)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-G78)-FRB
   

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23a_mod_RBPMS_FL

    Plasmid

    #59393

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    6xHis, FLAG, and HA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. mKikGR-Tubulin-6

    Plasmid

    #57330

    Purpose

    Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mKikGR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 Cas9-HMGB1

    Plasmid

    #183198

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-HMGB1
    

    Use

    CRISPR; Gateway entry clone

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-B2UP49_GFP

    Plasmid

    #103071

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

    Plasmid

    #187273

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    Anillin A740D, E758K

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 Cas9-RFC4

    Plasmid

    #183199

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC4
    

    Use

    CRISPR; Gateway entry clone

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEL062

    Plasmid

    #124894

    Purpose

    For genome editing in plant by SpCas9 wild type

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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