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Showing: 11541 - 11560 of 68611 results

  1. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120808-plasmid-map-sequence-id-235136

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  4. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  6. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Publication
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162277-plasmid-map-sequence-id-319794

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  9. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

    Plasmid
    #247160
    Purpose
    Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Puromycin resistance, mCherry
    Expression
    Mammalian
    Promoter
    Ef1a
    Available since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247160-plasmid-map-sequence-id-492681

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  15. pET29b-rhMOG

    Plasmid
    #225484
    Purpose
    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli
    Depositor
    John Pak
    Publication
    Wu et al J Immunol Methods. 2024 Oct 13:113764. d
    Insert
    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-29 (signal peptide) and 150…
    Promoter
    T7
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225484-plasmid-map-sequence-id-449491

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  19. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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Showing: 11541 - 11560 of 68611 results