We narrowed to 19,455 results for: Kin

1. LENTICRISPR-TPI1_sgRNA2

   Plasmid

   #201627

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   TPI1 (TPI1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-puro SHP2 D61A

   Plasmid

   #8330

   Depositor

   Ben Neel

   Article

   Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D61A (activated mutant)

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-C3-PLK4-S305A-3xFLAG

   Plasmid

   #69839

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Use

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305A

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN HA-GBRP

   Plasmid

   #89296

   Purpose

   For expression of the mammalian Atg8 protein GABARAP tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

5. inhibin-DsRed

   Plasmid

   #156184

   Purpose

   Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

   Depositor

   Goro Yoshizaki

   Article

   Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

   Inserts

   rainbow trout inhibin α promoter
   rainbow trout β actin 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   inhibin

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b GFP-Rab10 Q68LHis

   Plasmid

   #186015

   Purpose

   GFP-Rab10 Q68LHis

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   GFP-Rab10 Q68LHis (RAB10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac_Dual_GST-TEV-EGFP-TBK1

   Plasmid

   #187830

   Purpose

   Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST and TEV-mEGFP-GG Linker

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

   Plasmid

   #117384

   Purpose

   An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-CRY2

    Plasmid

    #140572

    Purpose

    For use in light-induced protein inactivation through the interaction with CIB1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mGnptg

    Plasmid

    #78106

    Purpose

    Mammalian expression of mouse Gnptg with C-terminal His tag

    Depositor

    Thomas Braulke

    Article

    Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

    Insert

    Gnptg (Gnptg Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pYFP-NGAT(A193T,N194Y)

    Plasmid

    #11388

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Changed Alanine 193 to Threonine, and Asparagine …

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-hMeCP2-GFP-R270X

    Plasmid

    #48079

    Purpose

    Mammalian expression of GFP tagged MeCP2-R270X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    expresses AT-Hook R270X

    Promoter

    CMV

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

    Plasmid

    #78516

    Purpose

    Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-Cx32-7

    Plasmid

    #54054

    Purpose

    Localization: Gap Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fort et al J Biol Chem. 2011 Jul 1;286(26):22875-85

    Insert

    Cx32 (GJB1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pRK5 Fyn deltaSH3

    Plasmid

    #16034

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta SH3: deletion of amino acids 76-141

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pMX-IY LC3C

    Plasmid

    #89292

    Purpose

    For expression of the mammalian Atg8 protein LC3C without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSOMA-T679A-NLS

    Plasmid

    #118938

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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