We narrowed to 25,458 results for: nes
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Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2
Plasmid#192440PurposeEncodes mCherry-tagged wild-type TSC2 isoform 5.DepositorInsertmCherry-TSC2 (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOGG012
Plasmid#113986PurposeVector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectorsDepositorInsertPartition genes (parABCDE)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK-RetE1-Citrine
Plasmid#127779PurposeEnhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activityDepositorInsertRetE1
UseEnhancer reporter constructAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-dCas9
Plasmid#84506PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64DepositorInsertV2-MESA-35F-M-dCas9
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STMN1
Plasmid#31326DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
ExpressionMammalianAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS52-V5-6His
Plasmid#175096PurposeV5 tagged VPS52 is expressed in mammalian cellsDepositorInsertVPS52 (VPS52 Human)
TagsV5ExpressionMammalianMutationN666D- please see depositor commentAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sox1/2/3-2373/+3>nls::Cas9::nls
Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRPromoterPtetAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 beta-catenin Y654F
Plasmid#16072DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2AD273K
Plasmid#202727Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)
ExpressionMammalianAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Lyn11C32S-PIP4K2A
Plasmid#202748Purposeweakly plasma membrane targeted fluorescent protein-tagged enzymeDepositorInsertEGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FBL6-FLAG3C
Plasmid#19904DepositorAvailable SinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-VHH[GFP]
Plasmid#163856PurposeZebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRXDepositorInsertVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2Available SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-CD44b-TurboID
Plasmid#163850PurposeIn vitro transcription of CD44b-TurboID. Parton lab clone JTJDepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX230
Plasmid#29479DepositorAvailable SinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ210
Plasmid#109329PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selectionDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1511
Plasmid#91037PurposeModule A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9
UseCRISPRPromoterOsAct1Available SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only