We narrowed to 5,049 results for: golden gate
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Plasmid#229322PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_sfGFP_PartType9DepositorInsertsfGFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK086 [CDS_CFP_PartType9]
Plasmid#229323PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_CFP_PartType9DepositorInsertCFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK087 [CDS_EBFP2_PartType9]
Plasmid#229324PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_EBFP2_PartType9DepositorInsertEBFP2
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK088 [CDS_GFP_PartType9]
Plasmid#229325PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_GFP_PartType9DepositorInsertGFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK089 [CDS_mBlueberry2_PartType9]
Plasmid#229326PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mBlueberry2_PartType9DepositorInsertmBlueberry2
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK090 [CDS_mCerulean_PartType9]
Plasmid#229327PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mCerulean_PartType9DepositorInsertmCerulean
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK091 [CDS_mKalama1_PartType9]
Plasmid#229328PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mKalama1_PartType9DepositorInsertmKalama1
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK074 [CDS_mRFP_PartType6]
Plasmid#229311PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mRFP_PartType6DepositorInsertmRFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK075 [CDS_sfGFP_PartType6]
Plasmid#229312PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_sfGFP_PartType6DepositorInsertsfGFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VPR
Plasmid#231572PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VPR, Ef1a driven
ExpressionMammalianAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA26_sgRNA-A0_M13ps
Plasmid#231413PurposeM13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).DepositorInsertsgRNA-A0
UseSynthetic BiologyAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAF23
Plasmid#223171PurposeExpresses yeGFP under control of CTR1 promoter and terminatorDepositorInsertgreen fluorescent protein
ExpressionYeastPromoterSaccharomyces cerevisiae CTR1Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAF24
Plasmid#223172PurposeExpresses VC83_00191-yeGFP under control of CTR1 promoter and terminatorDepositorInsertCopper Transporter
TagsStreptagII and yeGFPExpressionYeastPromotersaccharomyces cerevisiae CTR1Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
cpLOV2-Gal4
Plasmid#231569PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertcpLOV2-Gal4
ExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VP64
Plasmid#231571PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VP64
ExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR
Plasmid#231573PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VPR, CMV driven
ExpressionMammalianAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK83
Plasmid#226793PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10BDepositorInsertslr0214 (slr0214 Synechocystis sp. PCC 6803)
ExpressionBacterialPromoterTetracycline resistance cassette promoter (consti…Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOM034_P7_3"Lys3
Plasmid#216459Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM038_P8b_5'Lys3
Plasmid#216463Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB67
Plasmid#226316PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with K to A CsoS2 MR1-6
ExpressionBacterialMutationK261A, K320A, K380A, K436A, K495A, K546APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB74
Plasmid#226314PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-5
ExpressionBacterialMutationY300A, Y359A, Y420A, Y475A, Y534APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB73
Plasmid#226313PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-3
ExpressionBacterialMutationY300A, Y359A, Y420APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB72
Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
ExpressionBacterialMutationY300APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB45
Plasmid#226304PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-2
ExpressionBacterialMutationC292S, C310S, C351S, C369SPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
ExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB43
Plasmid#226302PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertWT CsoS2, frameshift site intact
ExpressionBacterialPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1426
Plasmid#218622PurposeExpress tNCS to the peroxisome for toxicity assayDepositorInserttNCS
TagsYFP-ePTS1ExpressionYeastMutation106-588Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1462
Plasmid#218623PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV proteaseDepositorInsertYFP
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1463
Plasmid#218624PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV proteaseDepositorInsertYFP
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1465
Plasmid#218625PurposeExpress engineered TFs Adr1c(S230A), Pip2c, and Oaf1cDepositorInsertAdr1
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1734
Plasmid#218627PurposeExpress amorphadiene synthase (ADS)DepositorInsertADS
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1401
Plasmid#218615PurposeExpress Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12DepositorInsertPex13
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB207
Plasmid#218596PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
TagsUbiY degradation tag and ePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1469
Plasmid#218588PurposeExpress peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonateDepositorInsertMvaS
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMVN
Plasmid#223861Purpose4G-cloning donor for mammalian promoter and terminator, N-terminal tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5417_p3WJdB_promoter dropout vector
Plasmid#221191PurposePromoter probe vector with dropout sequence for promoter sequences with 3WJdB reporterDepositorTypeEmpty backboneUseSynthetic BiologyPromoterPromoter Placeholder/Dropout VectorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_phN
Plasmid#223858Purpose4G-cloning donor for insect promoter and terminator, N-terminal tag addedDepositorInsertpolyhedrin promoter and SV40 polyA
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-TS-His10
Plasmid#223893Purpose4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tagDepositorInsert3C-TwinStrep-His10
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_Avi-3C-TS
Plasmid#223894Purpose4G-cloning donor for C-terminal AviTag-3C-TwinStrep tagDepositorInsertAviTag-3C-TwinStrep
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-GST-TEV
Plasmid#223878Purpose4G-cloning donor for N-terminal His6-GST-TEV tagDepositorInsertHis6-GST-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-MBP-TEV
Plasmid#223877Purpose4G-cloning donor for N-terminal His6-MBP-TEV tagDepositorInsertHis6-MBP-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-HA2-TEV
Plasmid#223876Purpose4G-cloning donor for N-terminal His6-HA2-TEV tagDepositorInsertHis6-HA2-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_3'omega
Plasmid#223870Purpose4G-cloning donor for 3'-Gibson overhang, omega-sequenceDepositorInsert3'omega sequence and spacer
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6
Plasmid#223871Purpose4G-cloning donor for N-terminal His6 tagDepositorInsertHis6
UseGolden-gate donorTags6xHisAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-TEV
Plasmid#223872Purpose4G-cloning donor for N-terminal His6-TEV tagDepositorInsertHis6-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-3C
Plasmid#223873Purpose4G-cloning donor for N-terminal His6-3C tagDepositorInsertHis6-3C
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3
Plasmid#223874Purpose4G-cloning donor for N-terminal His6-FLAG3 tagDepositorInsertHis6-FLAG3
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3-TEV
Plasmid#223875Purpose4G-cloning donor for N-terminal His6-FLAG3-TEV tagDepositorInsertHis6-FLAG3-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only