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We narrowed to 4,045 results for: golden gate

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Showing: 2851 - 2900 of 4045 results

  1. pEPOR1CB0020

    Plasmid
    #117556
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117556-plasmid-map-sequence-id-228155

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  2. pEPOR1CB0019

    Plasmid
    #117555
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117555-plasmid-map-sequence-id-228154

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  3. pEPOR1CB0018

    Plasmid
    #117554
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117554-plasmid-map-sequence-id-228153

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  7. pTU2_2b

    Plasmid
    #73011
    Purpose
    Secondary module (BpiI) site in pTU2b backbone
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Secondary module + Golden Gate pTU2b BsmBI destination site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    73011-plasmid-map-sequence-id-133862

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  10. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Article
    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  11. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  13. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  19. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

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  29. PB-CT3G-ERP2

    Plasmid
    #175497
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    175497-plasmid-map-sequence-id-345451

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  33. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  35. pRAM28

    Plasmid
    #234314
    Purpose
    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications
    Depositor
    James Chappell
    Article
    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103
    Insert
    U1367 Targeting RAM, Barcode 2
    Use
    Synthetic Biology
    Promoter
    P. CymRC
    Available Since
    March 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234314-plasmid-map-sequence-id-466009

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  37. pC-GoldyTALEN

    Plasmid
    #38143
    Purpose
    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
    Depositor
    Daniel Carlson
    Article
    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.
    Insert
    GoldyTALEN
    Tags
    AcV5 and FokI homodimer
    Expression
    Mammalian
    Mutation
    Truncate at N and C terminous
    Promoter
    miniCaggs
    Available Since
    July 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    38143-plasmid-map-sequence-id-48254

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  46. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  48. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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Showing: 2851 - 2900 of 4045 results