-
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK plasmid
Plasmid#169744PurposeUnregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV plasmid
Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-GFP-Blast
Plasmid#135673PurposeDox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selectionDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid
Plasmid#169732PurposeEqualizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-H plasmid
Plasmid#169367PurposeEqualizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF3) target-miR(FF3)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-M plasmid
Plasmid#169731PurposeEqualizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-H plasmid with onboard mCherry cassette
Plasmid#169733PurposeEqualizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserttetR-P2A-eGFP-miR(FF3) target-miR(FF3)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-M plasmid with onboard mCherry cassette
Plasmid#169734PurposeEqualizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Incoherent feedforward (IFF) plasmid
Plasmid#169748PurposeStandalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.DepositorInsertmiR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid with onboard mCherry cassette
Plasmid#169741PurposeCMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette
Plasmid#169743PurposeCMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.DepositorInsertmScarlet-I
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK+ plasmid with onboard mCherry cassette
Plasmid#169745PurposePGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette
Plasmid#169737PurposeEqualizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-274+5G>A
Plasmid#198023PurposeLV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in EcuadorDepositorInsertCD3D c.274+5G>A
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MCS-P2A-sfGFP
Plasmid#74668PurposePlasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.DepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-275-2A>G
Plasmid#198024PurposeLV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in JapanDepositorInsertCD3D c.275-2A>G
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript alpha-SSeCKS
Plasmid#61295Purposecontains rat alpha SSeCKS cDNADepositorInsertalpha SSeCKS (Akap12 Rat)
UseTagsExpressionBacterialMutationcDNA with 1kb of 3' UTR and 150bp of 5'…PromoterT7Available sinceDec. 9, 2014AvailabilityAcademic Institutions and Nonprofits only