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pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Publication

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Publication

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PMXS-SLC2A3

Plasmid

#72877

Purpose

expresses SLC2A3 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pLVX-M-Flag-BAP1-C91A

Plasmid

#125841

Purpose

express mutant BAP1-C91A

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

C91A

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1(-)-Cygnet2.1

Plasmid

#19737

Depositor

Wolfgang Dostmann

Publication

Honda et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

Cygnet 2.1

Expression

Mammalian

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Publication

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Publication

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA4-Flag-XPA

Plasmid

#22895

Depositor

Aziz Sancar

Publication

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

XPA (XPA Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hRAD18-EGFP

Plasmid

#68824

Purpose

Expresses human RAD18 tagged with EGFP

Depositor

Satoshi Tateishi

Publication

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 tagged with EGFP (RAD18 Human)

Tags

EGFP

Expression

Mammalian

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 myc-RNF114

Plasmid

#58295

Purpose

mammalian expression of myc tagged RNF114

Depositor

Francesca Capon

Publication

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHYRSF1-02

Plasmid

#24765

Depositor

Hideo Iwai

Publication

Muona et al Nat Protoc. 2010 . 5(3):574-87.

Insert

NpuDnaE-IntC

Tags

GB1 and His

Expression

Bacterial

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GfaABC1D-pm-iATPSnFR1.0

Plasmid

#102552

Purpose

expresses membrane displayed ATP sensor in astrocytes

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pREP

Plasmid

#51491

Purpose

pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Rep/RepA

Use

Plant expression t-dna

Mutation

none

Promoter

2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHel3

Plasmid

#102961

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Publication

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Shuttle vector

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLSL

Plasmid

#51493

Purpose

T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Plant t-dna plasmid

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Publication

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-dGFP-JARID1C

Plasmid

#74776

Purpose

retroviral expression of JARID1C

Depositor

Giovanni Tonon

Publication

Rondinelli et al Nucleic Acids Res. 2015 Mar 11;43(5):256

Insert

JARID1C (KDM5C Human)

Use

Retroviral

Expression

Mammalian

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Publication

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Expression

Insect

Promoter

hsp70 promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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