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Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT/TO/HIS/FLAG/HA-HNRNPU
Plasmid#38068DepositorPublicationInsertHNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
TagsHIS/FLAG/HAExpressionMammalianAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneTagsCBP tag, HA, and streptagExpressionMammalianAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV2/6
Plasmid#240485PurposeAAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid proteinDepositorInsertAAV2 Rep and AAV6 Cap
UseAAVExpressionMammalianAvailable sinceJuly 25, 2025AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phDLX2-N174
Plasmid#60860Purpose2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoterDepositorAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7-VEE-OKS-iM
Plasmid#58972PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoter26S subgenomic promoter and IRESAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_TRE3G-LSD1-dCas9-P2A-mCherry
Plasmid#138462Purpose2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherryDepositorPublicationInsertLSD1
UseCRISPR and LentiviralExpressionMammalianPromoterTRE3GAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorPublicationInsertSpCas9
TagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAMD1-2
Plasmid#61564PurposeStandardized mini-Tn5 delivery plasmid, kanamycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DEST PINK1-V5 KD
Plasmid#13319DepositorInsertPINK1 (PINK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationKinase dead: K219A/D362A/D384AAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only