We narrowed to 21,719 results for: ESC;

1. His-GST-Cys-M87 Spastin

   Plasmid

   #184804

   Purpose

   Bacterial expression of M87 Spastin (A2C mutation)

   Depositor

   James Hurley

   Article

   Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

   Insert

   M87 Spastin (SPAST Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   A2C

   Promoter

   T7

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-VHH-tev-std

   Plasmid

   #109423

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221_SLC6A2

   Plasmid

   #131891

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

   Insert

   SLC6A2 (SLC6A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. LSB-hsa-miR-516b-5p

   Plasmid

   #103601

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-516b-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FusionRed-Dectin1A-C-10

   Plasmid

   #56111

   Purpose

   Localization: Membrane, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   Dectin1A (CLEC7A Human)
   

   Use

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MSCV N-term HA-FLAG puro BRD7

   Plasmid

   #41934

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BRD7 (BRD7 Human)
   

   Use

   Retroviral

   Tags

   HA-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pKT2/CAGXSP/PalmNanoLuc

   Plasmid

   #182969

   Purpose

   Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmNanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-LplA(AAG)

   Plasmid

   #61307

   Purpose

   Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Use

   Tags

   6xHis, Flag, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 PA-mCit-GW

   Plasmid

   #113448

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-P3-IgKL-mNG

    Plasmid

    #183465

    Purpose

    To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF11-wmiRFP2-T2A-HO1

    Plasmid

    #179485

    Purpose

    Expresses miRFP2 and HO1in worm cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    wmIRFP2-T2A-HO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    tag-168 promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    odr-3

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-hK + E2-Crimson

    Plasmid

    #74946

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px458_2A_GFP_sgRNA_LTN1

    Plasmid

    #127125

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA LTN1 (LTN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-p27 T187A Luc

    Plasmid

    #23048

    Depositor

    William Kaelin

    Article

    Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

    Insert

    p27 (CDKN1B Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    T187A: mutation at codon 187 changing from T (ACG…

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge