We narrowed to 9,435 results for: try

1. pD-MSP1D1

   Plasmid

   #161607

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   D-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans were replaced b…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131-tRNA2.0

   Plasmid

   #158393

   Purpose

   Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Without promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFP.E227

   Plasmid

   #138249

   Purpose

   Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   mCherry
   Terminator
   mTagBFP
   Terminator
   gfp
   Terminator
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P17_up1691, P20_992, and iP16_3622

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR83

   Plasmid

   #90211

   Purpose

   Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

   Inserts

   OmpR (ompR E. coli)
   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-M2-TLR2

   Plasmid

   #12291

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Use

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I and H202Y; signal peptide (aa1-23) replaced …

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   VP64 activator fusion; NLS fusion

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Tags

   Expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Promoter

   Available since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG327

    Plasmid

    #128797

    Purpose

    Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl(s)

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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