We narrowed to 13,858 results for: Cre

Showing: 11661 - 11680 of 13858 results

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pcDNA3_ATP10B-G671R-N865K

Plasmid

#203699

Purpose

expresses ATP10B with the pathogenic mutations G671R and N865K

Depositor

Peter Vangheluwe

Publication

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

G671R, N865K

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGA guide 1

Plasmid

#193610

Purpose

PIGA knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 1 (PIGA Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGA guide 2

Plasmid

#193611

Purpose

PIGA knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 2 (PIGA Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkBIA guide 1

Plasmid

#193595

Purpose

NFkBIA knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkBIA guide 1 (NFKBIA Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkBIA guide 2

Plasmid

#193596

Purpose

NFkBIA knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkBIA guide 2 (NFKBIA Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 2

Plasmid

#193601

Purpose

AP2M1 knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 2 (AP2M1 Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 2

Plasmid

#193592

Purpose

RELA knockout

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 2 (RELA Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1-287)

Plasmid

#182839

Purpose

CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

Depositor

Peter Harte

Publication

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

CBP1-287

Expression

Bacterial

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-nTEVp

Plasmid

#179670

Purpose

Mutant Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-cTEVp

Plasmid

#179671

Purpose

Mutant Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-PML peptide

Plasmid

#175247

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p23-NES-RanCas13b-msfGFP-NES-Flag

Plasmid

#165073

Purpose

overexpression of RanCas13b in human cells

Depositor

Ling-Ling Chen

Publication

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RanCas13b

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9996-HeF-ABE

Plasmid

#163004

Purpose

plasmid expressing HeF-ABE

Depositor

Ervin Welker

Publication

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HeF-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_S130A

Plasmid

#174399

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

S130A

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_Y167F

Plasmid

#174398

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Y167F

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cMYC-AIP2

Plasmid

#118667

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Publication

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AIP2 (AT5G20910 Mustard Weed)

Tags

Myc

Expression

Plant

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 3

Plasmid

#135359

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

Depositor

Alex Hewitt

Publication

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 CP nSpyCas9 ABEMax

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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