We narrowed to 19,744 results for: Cre

1. GFP-BRD7

   Plasmid

   #65379

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD7 (BRD7 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1.Gαs-LgB113

   Plasmid

   #134362

   Purpose

   Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαs-LgB113 (GNAS Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BRD3

   Plasmid

   #65377

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD3 (BRD3 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY093-EFS-BCMA-Blast-WPRE

   Plasmid

   #192194

   Purpose

   Lenti-BCMA-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Mutation

   NA

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLSec2-irisin-his

   Plasmid

   #122729

   Purpose

   Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Mouse, Rat, Human, Bovine)
   

   Tags

   his and signal sequence for secretion

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BACE2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176942

   Purpose

   Mammalian expression vector encoding BACE2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   BACE2 (BACE2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DOP1B_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176979

    Purpose

    Mammalian expression vector encoding DOP1B and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    DOP1B (DOPEY2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_CD63-SBP-mCherry

    Plasmid

    #222326

    Purpose

    Synchronize the trafficking of CD63 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMKLR1-DuET

    Plasmid

    #213212

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CMKLR1 (CMKLR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPRC6A-DuET

    Plasmid

    #213299

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-RAB10

    Plasmid

    #130883

    Purpose

    Expresses human RAB10 with N-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    RAB10 (RAB10 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-ASH1L

    Plasmid

    #65369

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ASH1L (ASH1L Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-24d-Flag-SKP1

    Plasmid

    #213690

    Purpose

    Expresses human SKP1 in bacteria cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1 (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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