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hu6-sgCebpa_v1 (opti)

Plasmid

#177245

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_1st

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpa_v2 (opti)

Plasmid

#177246

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_2nd

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v1 (opti)

Plasmid

#177247

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_1st

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v2 (opti)

Plasmid

#177248

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_2nd

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v1 (opti)

Plasmid

#177249

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_1st

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-pchD WT

Plasmid

#187973

Purpose

Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Publication

Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

Insert

pchD WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP30-CFP

Plasmid

#178304

Purpose

CoTrIP plasmid with HSP30 promoter driving CFP's expression

Depositor

Brian Zid

Publication

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP30pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP30 and NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP26-CFP

Plasmid

#178306

Purpose

CoTrIP plasmid with HSP26 promoter driving CFP's expression

Depositor

Brian Zid

Publication

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP26pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP26 and NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Publication

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Expression

Yeast

Promoter

GLC3 and NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Cavin4b in pBluescript(SK+)

Plasmid

#126566

Purpose

For generating RNA probes for in situ hybridisation. Parton lab clone IRZ

Depositor

Rob Parton

Publication

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Gateway Cloning

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD30

Plasmid

#173033

Purpose

pQE30 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Publication

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

S. flexneri 2a wzzBSF gene

Tags

6xHis

Expression

Bacterial

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#1

Plasmid

#170834

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#1

Plasmid

#163394

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#3

Plasmid

#163396

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB1

Plasmid

#168800

Purpose

Fluorogen activating protein (FAP) DiB1

Depositor

Alexander Mishin

Publication

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB1

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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