We narrowed to 27,246 results for: STI

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pGEX_hDDX5_FL_D248N

Plasmid

#88872

Purpose

inducible expression of GST-DDX5 D248N in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Tags

GST

Expression

Bacterial

Mutation

D248N

Promoter

Tac

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep170 C-term (Nigg GG2)

Plasmid

#41151

Depositor

Erich Nigg

Article

Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

Insert

Cep170 (CEP170 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains C-terminal fragment (aa 755-1460, from X…

Promoter

CMV, T7

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pCDH-EF1a-extGluc-NGFR

Plasmid

#104832

Purpose

extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

extGLuc
NGFR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3A

Plasmid

#66819

Purpose

dCas9 fused to BFP and the human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A

Tags

dSpCas9-BFP-DNMT3A

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Mecom 1

Plasmid

#118590

Purpose

Expresses Mecom isoform 1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Mecom (Mecom Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-His6

Plasmid

#223583

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

His6 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-dnTCF4-ires-GFP

Plasmid

#133378

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

dnTCF4 (TCF4 Human)

Tags

2x Flag

Expression

Mammalian

Mutation

deltaN31

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1424

Plasmid

#29220

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-EGFP-NLS

Use

High copy number homologous recombination plasmid…

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Plasmid

#101230

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL1-9155

Plasmid

#115460

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-9155 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLX311 HRAS

Plasmid

#117749

Purpose

Open reading frame vector encoding HRAS

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

RASH1 (HRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Closed vector so will not read through the V5 reg…

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

Plasmid

#165437

Purpose

Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Mutation

H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

Promoter

CMV

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) WT-HA

Plasmid

#199660

Purpose

Transient expression of LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1b

Plasmid

#176025

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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