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pX330_DAZL_cterm2

Plasmid

#222919

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm3

Plasmid

#222920

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 3 (DAZL Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm4

Plasmid

#222909

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 4 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm3

Plasmid

#222908

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 3 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm2

Plasmid

#222907

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 2 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm1

Plasmid

#222906

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 1 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-GAL4-PARP1-CAT-L713F

Plasmid

#175949

Purpose

Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

GAL4 DNA binding domain (GAL4 Budding Yeast)

Tags

6xHis tag

Expression

Bacterial

Mutation

L713F, V762A for the 'PARP1-CAT-L713F' …

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DLX5

Plasmid

#222927

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DLX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

DLX5 (DLX5 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-LHX8

Plasmid

#222921

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

LHX8 (LHX8 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-FAM13A

Plasmid

#185550

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

FAM13A gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCi

Plasmid

#214038

Purpose

Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCi

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPCaspase

Plasmid

#214042

Purpose

Bacterial expression plasmid for PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase

Plasmid

#214043

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-N-term

Plasmid

#214044

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 1-153

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-C-term

Plasmid

#214045

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 154-666

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi

Plasmid

#214049

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-PCi

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PC-σ

Plasmid

#214037

Purpose

Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PC-σ

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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