We narrowed to 2,231 results for: D10

1. pnxABE-flgn

   Plasmid

   #187932

   Purpose

   A-to-G base editor

   Depositor

   Yi Xiao

   Article

   et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

   Insert

   ecTadA(8e)-nxCas9 (D10A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35S-PBE

   Plasmid

   #119772

   Purpose

   Conversion of C to T in potato

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   NLS-APOBEC1-nCas9-UGI-NLS
   

   Tags

   NLS

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   CaMv 2x35S

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2431

   Plasmid

   #36830

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Right (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. NAX_CAGGS_CAS9_m4_Puro

   Plasmid

   #167871

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SlugBE4max

   Plasmid

   #163799

   Purpose

   Expresses AncBE4max and nSlugCas9

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   SlugCas9 (D10A)

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. JEH127

   Plasmid

   #179299

   Purpose

   Expresses dCas9 fused to VPR

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VPR
   

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUHD Cdk2 WT HA

   Plasmid

   #27650

   Depositor

   Greg Enders

   Article

   Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

   Insert

   Cdk2 (CDK2 Human)
   

   Tags

   HA (YDVPDYA)

   Expression

   Mammalian

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQTEV-STX10

   Plasmid

   #31572

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   syntaxin 10 (STX10 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4095420

    Plasmid

    #149523

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-745 d1063-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_A2S-8

    Plasmid

    #188654

    Purpose

    AAV vector expressing SaAID-2S and gRNA

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S--nSaCas9-UGI
    Sa-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    Scp1 and U6

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_m4_EYFP

    Plasmid

    #167866

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4095421

    Plasmid

    #149524

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-745 d1063-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. BPK1160

    Plasmid

    #179298

    Purpose

    Expresses dCas9 fused to P65

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-p65
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUHD Cdk2 DN HA

    Plasmid

    #27651

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    D146N (Dominant Negative)

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. XE42 Rat frizzled-2-CS2+

    Plasmid

    #16814

    Depositor

    Randall Moon

    Article

    Yang-Snyder et al Curr Biol. 1996 Oct 1. 6(10):1302-6.

    Insert

    rat frizzled 2 (Fzd2 Rat)
    

    Use

    Xenopus expression

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid

    #242659

    Purpose

    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs

    Depositor

    Benjamin Kleinstiver

    Insert

    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Mutation

    SpCas9(S55R)

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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