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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pH1R-mCherry-N1

Plasmid

#84325

Purpose

Histamine-1 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R (HRH1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

Plasmid

#69625

Purpose

A FRET sensor for Galphai3 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2

Use

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pH1R-P2A-mCherry-N1

Plasmid

#84330

Purpose

Co-expresses (untagged) Histamine-1 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R-P2A-mCherry (HRH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-3xnls-Tq-Ca-FLITS

Plasmid

#145030

Purpose

piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Tags

3xnls-Tq-Ca-FLITS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pH4R-mCherry-N1

Plasmid

#84328

Purpose

Histamine-4 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-mCherry (HRH4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pH2R-P2A-mCherry-N1

Plasmid

#84331

Purpose

Co-expresses (untagged) Histamine-2 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R-P2A-mCherry (HRH2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)

Use

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Golgi

Plasmid

#36205

Purpose

In vivo visualization of golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Golgi targeting sequence (B4GALT1 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Mito

Plasmid

#36208

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

MTS (COX8A Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Use

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Use

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV,&Rhamnose

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lck-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129627

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Lck-cpmTq2-Calcium-lifetime-sensor

Use

Tags

Lck

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A-L325R

Plasmid

#145373

Purpose

expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

Depositor

Hugues Abriel

Article

Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

Expression

Mammalian

Mutation

changed Leucine 325 to Arginine

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-CDC42-G12V-deltaCAAX

Plasmid

#176094

Purpose

Constitutively active, nuclear localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

CDC42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

Plasmid

#145027

Purpose

Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Lentiviral

Tags

3xnls-Tq-Ca-FLITS and H2B-Maroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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