Addgene

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pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

Plasmid

#139844

Purpose

Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK-Kinase-dead (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Expression

Mutation

K47R

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2

Plasmid

#113945

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.b

Plasmid

#66588

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pLux01

Plasmid

#78281

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.a

Plasmid

#66587

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pLux02

Plasmid

#78282

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(E17K)

Plasmid

#86563

Purpose

Expresses human full-length Akt1(E17K) mutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

E17K mutation in the PH domain

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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J23100-mRFP1-331Bb

Plasmid

#78271

Purpose

mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23100-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168170

Purpose

Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL1

Plasmid

#109018

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV Syn ChR E90R-D156N-T159C 2A tDimer

Plasmid

#52494

Purpose

Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Depositor

Thomas Oertner

Article

Wietek et al Science. 2014 Mar 27.

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E90R, D156N, T159C

Promoter

human synapsin-1 and ribosomal skip sequence

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 ChR2 ET-TC 2A tDimer

Plasmid

#101361

Purpose

High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
synaptophysin-red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E123T , T159C

Promoter

human Synapsin

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only