We narrowed to 1,479 results for: pbluescript

1. pBS-IT5

   Plasmid

   #66869

   Purpose

   Inntag 5: a small self-structured epitope aimed at innocuous protein tagging

   Depositor

   Marti Aldea

   Article

   Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   Inntag 5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Spy EMX1-pegRNA

   Plasmid

   #169855

   Purpose

   SpyCas9-pegRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   Spy EMX1-pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJC30X-BS(X)

   Plasmid

   #58958

   Purpose

   Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

   Depositor

   Thomas Fox

   Article

   Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

   Insert

   Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
   

   Use

   Tags

   First 8 codons of COX3

   Expression

   Mutation

   GFP modified as described: CORMACK, B. P., R. H. …

   Promoter

   n/a

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. SauCas9 EMX1-nicking sgRNA

   Plasmid

   #169860

   Purpose

   SauCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

   Plasmid

   #52539

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA-G-intron-G

   Plasmid

   #40027

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP exon 1
   GFP exon 2
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of a loxP site, nucleotides 1-274, and …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT344_pattB-pCA-GFP-pA-FRT5

   Plasmid

   #52550

   Purpose

   for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GOMO-GFP-LUC

   Plasmid

   #60976

   Purpose

   Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

   Depositor

   Olivier Silvie

   Article

   Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

   Inserts

   GFP-Luciferase fusion
   mCherry
   hDHFR-yFCU
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Foxn1-pBSII SK

   Plasmid

   #41032

   Depositor

   Nancy Manley

   Article

   Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

   Insert

   Foxn1 in situ probe (Foxn1 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   T7 sense; T3 antisense

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTV-Nanog Bgeo

    Plasmid

    #13837

    Depositor

    Shinya Yamanaka

    Article

    Mitsui et al Cell. 2003 May 30. 113(5):631-42.

    Insert

    IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Nanog Mouse)
    

    Use

    Beta-gal

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBS ColIII alpha1

    Plasmid

    #19187

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Procollagen type III alpha 1 (COL3A1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pBS GDF10

    Plasmid

    #19186

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Growth differentiation factor 10 precursor (GDF10 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pKS913.CB18.31

    Plasmid

    #69569

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-1 mice

    Depositor

    Francis R. Carbone

    Article

    Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

    Insert

    TCR beta (Tcrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-TRE-mTagBFP2-WPRE

    Plasmid

    #193332

    Purpose

    Expresses mTagBFP2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A XhoI site in the coding sequence is silenced

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBstSK+IRES-V5-IPpoI (mutant)

    Plasmid

    #206322

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag-mutated IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein (mutant version)

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTV-Nanog Hyg

    Plasmid

    #13836

    Depositor

    Shinya Yamanaka

    Article

    Mitsui et al Cell. 2003 May 30. 113(5):631-42.

    Insert

    IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)
    

    Use

    Beta-gal

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pJRTCLCrVA010

    Plasmid

    #22843

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    A DNA containing GFP
    

    Use

    Cotton expression vector

    Tags

    Expression

    Bacterial

    Mutation

    Cotton Leaf Crumple Virus A component with Coat P…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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