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We narrowed to 60,327 results for: cloning

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Showing: 11781 - 11800 of 60327 results

  1. pLV-Hygro_hCOL4A3

    Plasmid
    #198098
    Purpose
    lentiviral expression of hCOL4A3 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A3 (COL4A3 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    198098-plasmid-map-sequence-id-467424

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  2. pKG141

    Plasmid
    #69759
    Purpose
    Retroviral expression of CENP-B
    Depositor
    Iain Cheeseman
    Insert
    CENP-B (CENPB Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. RECQL

    Plasmid
    #38890
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RECQL (RECQL Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    S611G
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38890-plasmid-map-sequence-id-178339

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  4. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  5. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

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  6. pCAG/TSF-VPS34

    Plasmid
    #232916
    Purpose
    Mammalian Expression
    Depositor
    James Hurley
    Insert
    VPS34 (PIK3C3 Human)
    Expression
    Mammalian
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232916-plasmid-map-sequence-id-465438

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  7. pLIX403-EBFP2-ccdB-G418

    Plasmid
    #158556
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158556-plasmid-map-sequence-id-309869

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  8. TXNRD1

    Plasmid
    #38863
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TXNRD1 (Txnrd1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38863-plasmid-map-sequence-id-271719

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  9. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80811-plasmid-map-sequence-id-152800

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  10. pAG416-GAL-RNQ1

    Plasmid
    #181706
    Purpose
    Galactose inducible expression of RNQ1
    Depositor
    Alejandro Chavez
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Expression
    Yeast
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181706-plasmid-map-sequence-id-430947

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  11. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  12. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  13. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  14. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  15. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  17. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. SYNJ1_BirA_Nterm

    Plasmid
    #221523
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Tags
    BirA
    Expression
    Mammalian
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221523-plasmid-map-sequence-id-439225

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  19. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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  20. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    177208-plasmid-map-sequence-id-356946

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Showing: 11781 - 11800 of 60327 results