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scFLARE1

Plasmid

#158738

Purpose

Mammalian expression of scFLARE1 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PM-RA

Plasmid

#209878

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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LD-RA

Plasmid

#209863

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cav-B

Plasmid

#209879

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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SAC1deltaTMD-FKBP-mCherry

Plasmid

#108123

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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15x-QUAS-dTomato-T2A-GCaMP6s

Plasmid

#130666

Purpose

QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

15xQUAS-dTomato-T2A-GCaMP6s

Use

Ae. aegypti expression

Expression

Insect

Promoter

15xQUAS-TATA

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMM134

Plasmid

#127215

Purpose

BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, IPT, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTC223

Plasmid

#70019

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230016

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

Lentiviral

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-TM

Plasmid

#82540

Purpose

Lentiviral vector for expressing HRP-TM in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

HRP fused to TM domain of PDGFR

Use

Lentiviral

Tags

HRP

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-anti-CD3/anti-CD19 BiTE

Plasmid

#230005

Purpose

Gal4-dependent expression of secreted BiTE

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-anti-CD3/anti-CD19 BiTE

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DeltaFosB

Plasmid

#68545

Purpose

AAV expression of DeltaFosB

Depositor

Eric Nestler

Article

Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

Insert

DeltaFosB-IRES-GFP

Use

AAV

Expression

Mammalian

Mutation

truncated splice variant of FosB

Promoter

CMV

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only