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We narrowed to 60,327 results for: cloning

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Showing: 11801 - 11820 of 60327 results

  1. SPOCK2_22-424_AviN_HisC

    Plasmid
    #195877
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK2 (SPOCK2 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Promoter
    Polyhedrin
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195877-plasmid-map-sequence-id-387267

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  2. PKMYT1

    Plasmid
    #39061
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKMYT1 (PKMYT1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pCWXPGR-pTF-Kir2.1

    Plasmid
    #114284
    Purpose
    TET-inducible expression of the Kir2.1 protein
    Depositor
    Patrick Salmon
    Insert
    Kir2.1 (KCNJ2 Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114284-plasmid-map-sequence-id-218907

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  4. EWS-GFP

    Plasmid
    #164701
    Purpose
    Expression of EWS-GFP
    Depositor
    Cheryl Arrowsmith
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    AcGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164701-plasmid-map-sequence-id-327076

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  6. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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  7. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Promoter
    pT7
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  8. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  9. RGS2

    Plasmid
    #38812
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RGS2 (Rgs2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. ER-FAST-IRES-iRFP670

    Plasmid
    #216344
    Purpose
    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum
    Depositor
    Alison Tebo
    Insert
    PRL_pFAST-IRES-CANX_iRFP670
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available Since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216344-plasmid-map-sequence-id-427396

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  11. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

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  12. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  13. pMH-EBFP

    Plasmid
    #161714
    Purpose
    Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    161714-plasmid-map-sequence-id-319502

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  14. NNMT

    Plasmid
    #40734
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NNMT (NNMT Human)
    Tags
    His Thrombin
    Expression
    Bacterial
    Mutation
    K100A:E101A:E103A
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. Mek2

    Plasmid
    #40776
    Depositor
    Dustin Maly
    Insert
    MKK2 (MAP2K2 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available Since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. Minos ITR loxp MCS lox2272

    Plasmid
    #218983
    Purpose
    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site
    Depositor
    Amro Hamdoun
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218983-plasmid-map-sequence-id-436166

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  17. pScarlessHD-3xFLAG-DsRed

    Plasmid
    #80820
    Purpose
    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    3xFLAG-piggyBac-DsRed
    Use
    Unspecified
    Tags
    3xFLAG
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80820-plasmid-map-sequence-id-152798

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  18. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  19. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Tags
    NLS
    Expression
    Insect
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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  20. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

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Showing: 11801 - 11820 of 60327 results