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pCAG_TWIST1

Plasmid

#215027

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTransposon.CAG.CasRX-P2A-Blast

Plasmid

#212965

Purpose

Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

CasRx

Use

Transposon

Tags

P2A-Blasticidin resistance

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-5-SV40 polyA

Plasmid

#190869

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode5

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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OpenCRISPR-1 sgRNA-16nt stem

Plasmid

#221566

Purpose

Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 16nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-EGFP barcode-1-SV40 polyA

Plasmid

#190865

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode1

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-0-SV40 polyA

Plasmid

#190864

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode0

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-3-SV40 polyA

Plasmid

#190867

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode3

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-4-SV40 polyA

Plasmid

#190868

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode4

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-2-SV40 polyA

Plasmid

#190866

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode2

Use

AAV

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (wt)

Plasmid

#31151

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

WT

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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CBE-T1.46

Plasmid

#193284

Purpose

Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.46

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS

Plasmid

#86675

Purpose

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 wt

Plasmid

#31663

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.52

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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CBE-T1.14

Plasmid

#193277

Purpose

Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.14

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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HMA-APOBEC1-Nb

Plasmid

#226012

Purpose

For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

Depositor

Eugene Yeo

Article

Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

Insert

APOBEC1 (Apobec1 Rat)

Tags

His-MBP and antiRabbit-nanobody

Expression

Bacterial

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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